We offer a full range of reliable IVF programs
OUR SERVICES INCLUDE:
— in vitro fertilization,
— intracytoplasmic sperm injection (ICSI),
— assisted hatching,
— intrauterine insemination (IUI),
— preimplantation genetic diagnosis,
— frozen embryo transfer.
In terms of effectiveness, our clinics can be compared with clinics in the US and Western Europe. The treatment is carried out by nationally recognized fertility experts.
TREATMENT PROGRAMS ARE AIMED TO SOLVE THE FOLLOWING PROBLEMS:
— low number of spermatozoa,
— pathology of the uterus or fallopian tubes,
— ovulation disorders,
— abnormalities during pregnancy caused by antibodies dangerous for the fetus,
— unexplained infertility,
— sperm malformations resulting in decreased egg penetration or cervical mucus survival.
Depending on your health problems, IFS will select the best medical clinics in Alma-Ata, which develop the most effective treatment programs. The whole treatment requires well-coordinated actions of medical staff and laboratories aimed at achieving pregnancy.
IVF in the US, Canada or Europe is much more expensive. We offer the most affordable prices for IVF.
HOW IT WORKS
The procedure of in vitro fertilization (further IVF) consists of the following stages:
—selection and examination of patients,
— superovulation induction with monitoring of folliculogenesis and development of the endometrium,
—puncture of ovarian follicles,
— fertilization of oocytes with sperm and cultivation of embryos in vitro,
— embryo transfer in the uterine cavity,
—luteal phase support,
—diagnosis of early pregnancy.
Puncture of ovarian follicles
Several eggs increase the probability of pregnancy. Normally, during menstrual cycle women produce only one egg. In order to obtain several eggs, doctors perform so-called superovulation stimulation, which takes 7 to 20 days and represents injections of hormonal preparations or their reception in tablet form.
The mode of administration of superovulation inductors is called a stimulation scheme or protocol. There are several schemes for stimulating superovulation, but the final amount, types and duration of administration are individual for each woman, and depend on her age, the cause of infertility and the follicular (ovarian) reserve.
Maturation of ovum cannot be traced by non-invasive methods, so it is measured indirectly by the growth of ovarian follicles. Growth of follicles is observed by ultrasound. When the dominant follicle reaches a certain size (16-20 mm), doctors prescribe transvaginal oocyte retrieval. It is performed under general (more often) or local (less often) anesthesia with the transvaginal needle, inserted under ultrasound guidance. Puncture is necessary to aspirate the follicular fluid, which is then examined under a microscope to identify oocytes.
Oocytes are washed from the follicular fluid and transferred to Petri dishes with a culture medium, which are placed in incubators while the sperm is prepared.
The patient produces the sperm through masturbation. If it is impossible to receive it by natural means, semen can be taken directly from the epididymis through surgical methods. Sperm is obtained either on the same day of the follicular puncture, or in advance with subsequent freezing and storage in liquid nitrogen. Before fertilization, the spermatozoa are washed from the seminal fluid and sorted using special methods to identify the qualitative ones.
If the patient does not have a sexual partner or his semen is unsuitable for fertilization, it is possible to use donor sperm. According to the Order of the acting minister of Health of the Republic of Kazakhstan from October 30, 2009, No. 624 “On Approval of the Rules for Donation and Storage of germ cells,” the donor sperm is quarantined and frozen for a period of six months and the donor is re-tested for infectious diseases prior to the sperm being used for artificial insemination.
Fertilization of oocytes in vitro with the husband / donor sperm
IVF is carried out by embryologists in the embryological laboratory.
The egg is fertilized in one of two ways:
1) in vitro,
2) with intracytoplasmic sperm injection (ICSI, ICSI).
The first method is easier, and involves combination of sperm with eggs in the culture medium at the rate of 100-200 thousand spermatozoa per egg. Within two or three hours, one of them fertilizes the egg. ICSI means the injection of the sperm into the egg with microsurgical instruments. The method is used when the sperm has a very poor quality and fertilization cannot be achieved in vitro.
The probability of successful fertilization is 60-70%. Upon penetration, the egg is considered an embryo. From two to six days embryos are kept in artificial conditions in CO2 incubators designed to maintain the atmosphere with 5-6% content of CO2 at a temperature of 37 ° C. Eggs and then embryos are stored in plastic dishes (Petri and Nunc dishes, plates, etc.) with the culture medium. It is composed of the main physiological ions (Na +, K +, Ca2 +, Mg2 +, Cl-, CO32-, etc.), energy substrates (glucose, pyruvate, lactate), amino acids, often vitamins and serum proteins. During the incubation period, the human embryo does not increase in size (in the first four days its size is 0.1 mm, on the fifth day is 0.15-0.2 mm). However, the number of its constituent cells increases many times (the first day – one cell, the second day – four cells, the third day – eight cells, the fourth day – from 10 to 20 cells, the fifth day – from 40 to 200 cells).
Transfer of the embryo to the uterus
The embryo is transferred to the uterus 2-5 days after the oocyte fertilization. The procedure takes a few minutes, does not require anesthesia and is performed on a gynecological chair. The embryo is transferred via a special elastic catheter inserted through the cervix. To avoid multiple pregnancies, no more than two embryos are usually inserted into the uterine cavity.